The Pantophlet Laboratory at Simon Fraser University is looking for a highly motivated and technically skilled Research Technician to assist with projects on antibody discovery, viral glycoprotein immunogens (including influenza, coronaviruses, and paramyxoviruses), and functional antibody analysis. The successful candidate will join a multidisciplinary research team working on molecular biology, mammalian cell culture, recombinant protein production, immunoassays, and flow cytometry. Appointment Type: Full-time, on-site Duration: 1-year term (3-month probationary period), with possibility of renewal subject to performance and funding Salary: CAD $65,00070,000 per year, commensurate with qualifications and experience, including benefits and statutory entitlements in accordance with SFU policies CORE RESPONSIBILITIES Mammalian Cell Culture & Expression Maintenance and passaging of adherent and suspension cell lines Transient transfection for recombinant protein expression Molecular Biology Cloning (e.g., Gibson assembly, T4 ligation) PCR and gene construct validation Site-directed mutagenesis (e.g., QuikChange) Plasmid DNA preparation (mini- and midipreps) Protein Production & Characterization Recombinant protein expression in mammalian systems Affinity purification (spin columns or gravity-based columns) SDS-PAGE and protein quantification (e.g., BCA assays) Immunoassays & Biophysical Analysis ELISA Biolayer interferometry (BLI) Data acquisition and analysis using instrument software Flow Cytometry Multi-color staining and assay setup Independent operation of BD flow cytometers (analyzer and sorter) Independent data analysis using FlowJo Additional Desirable Experience Antibody display techniques (e.g., yeast display) Automated nucleic acid synthesis platforms (e.g., BioXp) RNA quantification and quality assessment (e.g., Qubit-based assays) QUALIFICATIONS BSc (Honours preferred) or MSc in immunology, microbiology, molecular biology, or a related field Demonstrated hands-on experience with mammalian cell culture and molecular cloning Experience operating core research instrumentation (e.g., flow cytometry, BLI) Strong experimental organization and documentation skills Ability to work independently while contributing effectively within a structured research environment The ideal candidate will demonstrate experimental rigor, meticulous record-keeping, and the ability to perform established protocols consistently while identifying opportunities for improvement and troubleshooting. The appointment level will reflect experience; candidates showing a high degree of technical independence and troubleshooting skills will be considered for the Research Assistant/Technician III level. ELIGIBILITY Only Canadian citizens and permanent residents of Canada will be considered. APPLICATION REVIEW Applications will be reviewed immediately and will continue to be reviewed until the position is filled. Candidates should submit a single PDF including: A cover letter explaining how the role aligns with their career goals Curriculum vitae Names and contact details for three professional references (preferably supervisory)
Job Title
Research Technician / Research Assistant – Infectious Diseases Immunology