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Job Title: Sanger Sequencing SpecialistLocation: Hyderabad, Telangana Department: Molecular Biology & Gene TherapeuticsRole Overview:We are seeking a highly skilled and detail-oriented Sequencing Specialist with hands-on expertise and experience in Sanger sequencing. Experience in Next-Generation Sequencing (NGS) workflows and data analysis is a plus. The ideal candidate will be responsible for performing end-to end Sanger sequencing runs, also preparing NGS libraries, analyzing data, and ensuring accurate and timely delivery of results for internal and client projects.Key Responsibilities:Perform Sanger sequencing workflows, including PCR amplification, purification, and capillary electrophoresis etc.Prepare SOPs, engage in Sequencing method development, Operate and maintain sequencing instruments (e.g., ABI 3730xl for Sanger; Illumina or similar platforms for NGS).Ability to prepare NGS libraries for various applications (DNA, RNA, targeted panels, amplicon sequencing) for outsourcing.Perform quality control checks on libraries and sequencing data using tools like Bioanalyzer, Qubit, and qPCR.Analyze sequencing data: For Sanger: Interpret chromatograms, base calling, and sequence alignment.For NGS: Perform primary and secondary analysis (FASTQ QC, alignment, variant calling).Troubleshoot technical issues and optimize protocols for improved efficiency and accuracy.Maintain detailed documentation of experiments and results in compliance with laboratory standards.Collaborate with cross-functional teams to support molecular biology and genomics projects.Ensure adherence to quality control and safety guidelines in the laboratory.Participating in various laboratory operations and software development for sequencing requirements.End-to-end responsibility of maintaining the sequencing facility but not limited to ordering reagents & spare parts, calibration, PM, repair, training etc.Qualifications & Skills:Education: M.Sc./M. Tech in Molecular Biology, Biotechnology, or related field.Experience: 4-6 years of hands-on experience in Sanger sequencing and similar workflows.Experience in NGS library preparation and sequencing platforms (Illumina, Ion Torrent, etc.) is a plus.Familiarity with sequencing analysis software and bioinformatics tools (e.g., Chromas, Sequencing Analysis, FASTQC, BWA, GATK etc.).Strong understanding of PCR, primer design, and DNA/RNA purification techniques and equipment.Excellent troubleshooting and problem-solving skills.Ability to work independently and manage multiple projects simultaneously.Good communication and documentation skills is a must.Preferred:Experience with high-throughput sequencing and automation platforms.Knowledge of advanced bioinformatics pipelines for variant analysis and annotation.